Announcement PDF ABN 16 165 160 841 ASX RELEASE 21 May 2024 Change of Director’s Interest Notice – Appendix 3Y: Dr Robert Peach Appendix 3Y Change of Director’s Interest Notice for Dr. Robert Peach is attached. This ASX announcement was approved and authorised for release by the Company Secretary of Amplia Therapeutics Limited. - End - Investor Contact: Dr Chris Burns Chief Executive Officer chris@ampliatx.com Media Contact: H^CK Director, Haley Chartres haley@hck.digital +61 423 139 163 About Amplia Therapeutics Limited Amplia Therapeutics Limited is an Australian pharmaceutical company advancing a pipeline of Focal Adhesion Kinase (FAK) inhibitors for cancer and fibrosis. FAK is an increasingly important target in the field of cancer immunology and Amplia has a particular development focus in fibrotic cancers such as pancreatic cancer. FAK also plays a significant role in a number of chronic diseases, such as idiopathic pulmonary fibrosis (IPF). For more information visit www.ampliatx.com and follow Amplia on Twitter (@ampliatx) and LinkedIn. Level 17, 350 Queen St, Melbourne VIC 3000 Email info@ampliatx.com www.ampliatx.com Appendix 3Y Change of Director’s Interest Notice Appendix 3Y Rule 3.19A.2 Change of Director’s Interest Notice Information or documents not available now must be given to ASX as soon as available. Information and documents given to ASX become ASX’s property and may be made public. Introduced 30/09/01 Amended 01/01/11 Name of entity Amplia Therapeutics Limited ABN 16 165 160 841 We (the entity) give ASX the following information under listing rule 3.19A.2 and as agent for the director for the purposes of section 205G of the Corporations Act. Name of Director Dr. Robert Peach Date of last notice 2 January 2024 Part 1 - Change of director’s relevant interests in securities In the case of a trust, this includes interests in the trust made available by the responsible entity of the trust Note: In the case of a company, interests which come within paragraph (i) of the definition of “notifiable interest of a director” should be disclosed in this part. Direct or indirect interest Direct and Indirect Nature of indirect interest (including registered holder) Cofactor LLC of which Dr. Peach is a Director and owner Date of change 15 May 2024 No. of securities held prior to change Andrea Marie Peach and Robert James Peach <Peach Family Trust> - 331,808 Shares Note: Provide details of the circumstances giving rise to the relevant interest. Cofactor LLC – 1,332,952 Shares Cofactor LLC – 535,000 Unlisted Options Exercise Price A$0.26 Expiry Date 6 Sep 2025 Class Ordinary Shares Number acquired 1,900,005 shares Number disposed Nil Value/Consideration Entitlement Offer $0.055 per shares Note: If consideration is non-cash, provide details and estimated valuation + See chapter 19 for defined terms. 01/01/2011 Appendix 3Y Page 1 Appendix 3Y Change of Director’s Interest Notice No. of securities held after change Andrea Marie Peach and Robert James Peach <Peach Family Trust> - 2,231,813 Shares Cofactor LLC – 1,332,952 Shares Cofactor LLC – 535,000 Unlisted Options Exercise Price A$0.26 Expiry Date 6 Sep 2025 Nature of change Example: on-market trade, off-market trade, exercise of options, issue of securities under dividend reinvestment plan, participation in buyback Participation in Entitlement Offer as Sub-Underwriter Part 2 – Change of director’s interests in contracts Note: In the case of a company, interests which come within paragraph (ii) of the definition of “notifiable interest of a director” should be disclosed in this part. Detail of contract Nil Nature of interest Name of registered holder (if issued securities) Date of change No. and class of securities to which interest related prior to change Note: Details are only required for a contract in relation to which the interest has changed Interest acquired Interest disposed Value/Consideration Note: If consideration is non-cash, provide details and an estimated valuation Interest after change Part 3 – +Closed period Were the interests in the securities or contracts detailed above No traded during a +closed period where prior written clearance was required? If so, was prior written clearance provided to allow the trade to proceed during this period? If prior written clearance was provided, on what date was this provided? 21 May 2024 + See chapter 19 for defined terms. Appendix 3Y Page 2 01/01/2011